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Potentiometric biosensor based on biodegrading bacteriafor determination of methyl parathion Fangfang Geng1,2,Xuewei Wang1, Baojun Ding2, Wei Qin1* 1.Yantai Institute of Coastal Zone Research, Chinese Academy of Scinences, Yantai 264003, China 2.Dalian University of Technology, Dalian 116024, China Organophosphates pesticides (OPs) are widely used as agriculture insecticides. However,due to their neurotoxic effects on humans, the rapid and sensitive determination of OPs has become increasingly important.Here, a novel isolated bacterial strain, Klebsiella sp. MP-6, which is isolated from methyl parathion (MP) contaminated soil, is used tocatalyze the hydrolysis of MP to release p-nitrophenol[1]. Then, the product, which is related to the concentration of MP, is detected by a polymeric membrane ion-selective electrode [2]. The anionic responses owe to the extracted and complexed neutral phenols further underwent proton dissociation with concomitant ejection of HX from the membrane interface to the aqueous phase[3].Under the optimized conditions, the linear range is 0.05 - 1 μM in 10-4 M NaOHand the detection limit is 8.2 nM (S/N=3).
Keywords:Potentiometry, Methyl Parathion, Ion-selective electrode, Klebsiella, Biodegradation Acknowledgements This work was financially supported by the National Natural Science Foundation of China (Grant 41176081, Grant 21207156). References [1]. S. Wang, C. Zhang, Y. Yan, Biodegradation, 2012, 23, 107-116 [2]. X. Wang, Z. Ding, Q. Ren and W. Qin,Anal. Chem. 2013, 85, 1945-1950 [3]. X. Wang, D. Yue, E. Lv, L. Wu and W. Qin,Anal. Chem. 2014, 86, 1927-1931